File Name: hplc exam questions and answers .zip
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Solved examples with detailed answer description, explanation are given and it would be easy to understand. Here you can find objective type Biochemistry HPLC questions and answers for interview and entrance examination. Multiple choice and true or false type questions are also provided. You can easily solve all kind of Biochemistry questions based on HPLC by practicing the objective type exercises given below, also get shortcut methods to solve Biochemistry HPLC problems. All Rights Reserved. Contact us: info.
Are you engineering graduate? Searching for a job which gives good fetching and career growth? Are you trying for government jobs? This corporation is looking for young, dynamic and talented candidates who bring different ideas, skills and experiences to mix and ready to learn and dive into the organization. Put your efforts in getting job by looking into the HPCL interview questions and answers and face the future. It is a chromatographic technique used to separate the components in a mixture, to identify each component, and to quantify each component. In general, the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent.
Samples collected from medical patients, industry products, and the environment are usually mixtures of many compounds. Often times, doctors, producers, and researchers are interested in specific components in these mixtures, so these mixtures need to be separated. High-performance liquid chromatography HPLC offers the ability to do just that. HPLC data can be used to complement gas chromatography GC or be an excellent alternative to GC when the components are nonvolatile or would thermodynamically decompose under high temperatures. In order to separate mixture components, HPLC takes advantages of partitioning between a mobile and stationary phase under a uniform pressure that is typically between to psi. High pressure is required to obtain a reasonable flow rate through the column. The process begins when a small amount of liquid sample is injected into the column that has a stream of liquid flowing through which is known as the mobile phase.
What is interesting about science is that it is not "fixed" and continually is in a state of flux. Solved examples with detailed answer description, explanation are given and it would be easy to understand. Paper Chromatography of Gel Ink Pens It would be tempting to try to explain paper chromatography in terms of the way that different compounds are adsorbed to different extents on to the paper surface. This diagram from the Chemguide page shows the result of two way paper chromatography on a mixture of dyes. In such cases, there is no excuse not to have answers prepared. Question
Which of the following is the disadvantage of reciprocating pump used in liquid chromatography? Explanation: The disadvantage of reciprocating pump used in liquid chromatography is that it produces pulsed flow.
In reversed phase chromatography the mobile phase is 1 the stationary phase. The retention time is amongst others influenced by 7 strength of the organic modifier and 8. Rank the following compounds according to their adsorption ability in a decreasing order. Skip to content Multiple choice: Check your answers by clicking on them. Select the answers which apply for reversed-phase chromatography.
High Pressure Liquid Chromatography B. High Performance Liquid Chromatography C. Highly Placed Liquid Chromatography. For a typical adsorbent such as silica gel, the most popular pore diameters are.
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